Interaction of draxin and y-netrins

ABSTRACT

This invention relates to extracellular protein-protein interactions and their possible therapeutic uses. More particularly, this invention describes the interaction between Draxin, particularly fragments binding to γ-Netrins comprising SEQ ID NO.: 1, 2 or 3, and variants thereof, with γ-Netrins, and the use of this interaction to disrupt γ-Netrin/Netrin receptor interactions. The invention also relates to diagnostic and/or therapeutic uses of Draxin or fragments or variants thereof, as well as to an antibody against Draxin inhibiting binding of Draxin to γ-Netrins. Further, the invention relates to fragments of γ-Netrins, in particular Draxin-binding Netrin1-fragments comprising SEQ ID NO.: 51 and variants thereof, as well as to an antibody against γ-Netrins inhibiting binding of γ-Netrins to Netrin receptors.

FIELD OF THE INVENTION

This invention relates to extracellular protein-protein interactions and their possible therapeutic uses. More particularly, this invention describes the interaction between Draxin, particularly fragments binding to γ-Netrins comprising SEQ ID NO.:1, 2 or 3, and variants thereof, with γ-Netrins, and the use of this interaction to disrupt γ-Netrin/Netrin receptor interactions. The invention also relates to diagnostic and/or therapeutic uses of Draxin or fragments or variants thereof, as well as to an antibody against Draxin inhibiting binding of Draxin to γ-Netrins. Further, the invention relates to fragments of γ-Netrins, in particular Draxin-binding Netrin1-fragments comprising SEQ ID NO.: 51 and variants thereof, as well as to an antibody against γ-Netrins inhibiting binding of γ-Netrins to Netrin receptors.

BACKGROUND OF THE INVENTION

The laminin-related netrin protein family in humans comprises 5 members. Three of them, Netrin1, Netrin3, and Netrin4 are secreted proteins. NetrinG1 and NetrinG2 instead are linked to the cell surface by a GPI anchor. The LamNT domain and the EGF domains of Netrin1 and Netrin3 are derived from the γ chain of Laminin1. In the context of the present invention, these Netrins are thus referred to as “γ-Netrins”. In contrast, the corresponding domains of Netrin4, NetrinG1 and NetrinG2 are homologous to the domains present in the β chain of Laminin1 (Moore et al., 2007).

Netrin1 is a diffusible, laminin-related protein identified as neuronal guidance cue during development of the nervous system. Netrin1 mediates its biological effects through binding to receptors, which belong to the so-called dependence receptors, e.g. deleted in colorectal cancer (DCC) and uncoordinated-5-homolog (UNC5H). Recently, it has been found that Netrin1 is expressed outside the nervous system and contributes to the patterning of developing epithelial tissues such as mammary gland, pancreas, and lung by regulating diverse processes including adhesion, motility, proliferation, and differentiation of cells.

Numerous tumors have been described to express cell surface receptors belonging to the DCC-und UNC5-family. These receptors are binding to the secreted ligand Netrin1 in the extracellular space and serve, in addition to their well-established neurodevelopmental function, as dependence receptors in cancers (Castets et al., 2012; Mehlen et al., 2011). In tumors they can regulate tumor cell survival in a Netrin1 dependent manner. Netrin1 itself is known to be upregulated by many tumor types and has been suggested to act as an oncogene (Arakawa, 2004; Fitamant et al., 2008). If Netrin1 is not bound to dependence receptors of the DCC- and UNC5-family, the receptors cannot form dimers or multimers, which in turn triggers the activation of a pro-apoptotic pathway.

In several studies, decoy Netrin receptor fragments have been used to disrupt Netrin/Netrin receptor interactions in order to induce pro-apoptotic signaling. For example, such receptor fragments have been used in cancer cell lines (Delloye-Bourgeois et al., 2009; Fitamant et al., 2008) and in animal models (Fitamant et al., 2008; Paradisi et al., 2013; Paradisi et al., 2009) to induce cancer cell death. However, using a fragment of a Netrin receptor causes interference at a relatively late stage of the signaling cascade, namely just before dimerization of the receptor. Moreover, even when using high concentrations of these decoy receptors, a residual binding of Netrin to the full length receptor cannot be prevented.

It was thus an object of the invention to provide compounds that can be used for interfering with the binding of γ-Netrins, in particular Netrin1 to at least one of its receptors, which at least partially overcome the disadvantages of the prior art.

Draxin is a secreted protein described to be involved in axon guidance decisions (Islam et al., 2009). In contrast to Netrins 1-3, which are present in vertebrates and invertebrates, Draxin can only be found in vertebrate genomes. The amino acid sequence of human Draxin is shown as SEQ ID NO.: 4. In zebrafish, there exist two Draxin isoforms (DraxinA and DraxinB); their amino acid sequences are represented by SEQ ID NO.: 5 and SEQ ID NO.: 6.

By using an extracellular protein-protein interaction screen assay (AVEXIS), the present inventors identified Draxin as a novel direct binding partner for Netrin1. Furthermore, by using an AVEXIS based competition assay, the inventors were able to show that Draxin or Draxin protein fragments can compete with Netrin receptors for binding to Netrin1.

The present invention therefore provides specific peptides binding to γ-Netrins (“γ-Netrin-binding peptides”), particularly to Netrin1, as well as antibodies directed against γ-Netrins (“γ-Netrin-binding antibodies”), particularly against Netrin1, which may be used for interfering with γ-Netrin/Netrin receptor binding, in particular Netrin1/Netrin receptor binding. The invention further provides Draxin-binding peptides as well as Draxin-binding antibodies inhibiting binding of Draxin to γ-Netrins, in particular to Netrin1.

DETAILED DESCRIPTION OF THE INVENTION

In one aspect, the present invention relates to peptides which bind to at least one γ-Netrin, with a high specificity and a high affinity. Importantly, the affinity of Draxin to the γ-Netrins, in particular to Netrin1, is significantly higher than the affinity of γ-Netrins, in particular of Netrin1 to Netrin receptors.

Accordingly, the invention provides a γ-Netrin-binding peptide, comprising (i) the sequence EVMPTLDMALFDWTDYEDLKP (SEQ ID NO.: 1), or (ii) the sequence DVAPTFNMALFDWTDYEDMRP (SEQ ID NO.: 2), or (iii) the sequence EVMPTLDMTLFDWTDYEDMKP (SEQ ID NO.: 3), or (iv) a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.: 1, 2 and/or 3, wherein said peptide has a length of up to 328 amino acids and is optionally fused to a heterologous peptide or polypeptide. Preferably, the γ-Netrin is Netrin1.

The γ-Netrin-binding peptide is characterized in that it binds with high affinity to Netrins derived from the γ chain of Laminin1, comprising in particular human Netrin1 and human Netrin3 as well as Netrins 1a, 1b and 2 from zebrafish (Danio rerio). In particular, the γ-Netrin-binding peptide of the invention binds to human Netrin1 and to zebrafish Netrin1a (also referred to as Ntn1a) and Netrin1 b (also referred to as Ntn1b). In preferred embodiments, it is therefore referred to as “Netrin1-binding peptide”.

In some embodiments, the peptide with a length of up to 328 amino acids comprises any one of SEQ ID NO.: 1, 2, 3 or a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.: 1, 2 and/or 3 and is free of any further heterologous peptides.

In other embodiments, the peptide with a length of up to 328 amino acids comprises any one of SEQ ID NO.: 1, 2, 3 or a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.: 1, 2 and/or 3 and is fused to a heterologous peptide or polypeptide as defined below.

The minimal sequence of the γ-Netrin-binding peptide, in particular Netrin1-binding peptide is represented by

SEQ ID NO: 1 EVMPTLDMALFDWTDYEDLKP, SEQ ID NO.: 2 DVAPTFNMALFDWTDYEDMRP, and SEQ ID NO.: 3 EVMPTLDMTLFDWTDYEDMKP, respectively.

In some embodiments, the peptide has a length of up to 328 amino acids, up to 324 amino acids, up to 320 amino acids, up to 250 amino acids, up to 200 amino acids, up to 100 amino acids, up to 50 amino acids, or up to 30 amino acids. According to other embodiments, the peptide has a length of up to 150 amino acids or up to 125 amino acids. Fragments comprising up to 50 amino acids are preferred according to some embodiments.

In certain preferred embodiments, a variant of SEQ ID NO.: 1, SEQ ID NO.: 2 or SEQ ID NO.: 3 have a sequence identity of at least 70% to SEQ ID NO.: 1, 2 and/or 3. In further embodiments the level of sequence identity may be at least 90%, or even at least 95% to SEQ ID NO.: 1, 2 and/or 3.

A “variant” in the context of the present invention is any peptide whose amino acid sequence varies in at least one position from the respective reference peptide, but retains the biological activity of the reference peptide; for example, a variant of SEQ ID NO.: 1 differs in at least one amino acid therefrom, and retains the γ-Netrin-, particularly Netrin1-binding activity. In particular, variants of SEQ ID NO.: 1, 2 and 3 differ in 1, 2, 3, 4, 5, 6 or 7 amino acids from SEQ ID NO.: 1, SEQ ID NO.: 2 and/or SEQ ID NO.: 3, provided they retain the γ-Netrin-, particularly Netrin1-binding activity. Variations will usually be generated by amino acid substitutions. Particularly, a variant according to the invention will be characterized in that it has been changed to contain at least one non-naturally occurring substitution modification relative to the respective reference peptide.

The peptidic compounds of the present invention comprise a linear backbone of amino carboxylic acids linked by peptide, i.e. carboxamide bonds. Preferably, the amino carboxylic acids are α-amino carboxylic acids and more preferably L-α-amino carboxylic acids, unless indicated otherwise. Any amino acid of the sequences disclosed herein may be replaced either by an unmodified canonical proteinogenic L-amino acid, or by an unmodified canonical proteinogenic D-amino acid. Also envisaged are substitutions with non-canonical proteinogenic amino acids, in particular with ornithine, 2,4-diamino butyric acid, 2,3-diamino propionic acid, selenocysteine, pyrrolysine, hydroxyproline, O-phosphoserine, O-phosphotyrosin, γ-carboxyglutamic acid, γ-aminobutyric acid, norleucine, ε-aminohexanoic acid, and with other posttranslationally modified amino acids, e.g. amino acids with amidated carboxyl groups (at C-termini of peptides), amino acids with alkylated (e.g. methylated) side chains, amino acids with an amino side chain group (such as lysine and ornithine) with modifications at one or both of the hydrogen atoms of the amino side chain group, for example with a lipophilic moiety attached via a carboxamide bond, etc.

The percent sequence identity may be determined according to the following formula:

I=n:L

wherein I is the identity in percent, n is the number of identical amino acids between a given sequence and a comparative sequence as shown e.g. in SEQ ID NOs.: 1, 2 and 3, and L is the length of the comparative sequence. Importantly, when calculating the percent sequence identity according to this formula, an alignment of the two sequences shall be carried out without gaps between complementary portions and over the whole length of the comparative sequence.

In specific embodiments, the invention provides a γ-Netrin-binding peptide, comprising (i) the sequence EVMPTLDMALFDWTDYEDLKP (SEQ ID NO.: 1), or (ii) the sequence DVAPTFNMALFDWTDYEDMRP (SEQ ID NO.: 2), or (iii) the sequence EVMPTLDMTLFDWTDYEDMKP (SEQ ID NO.: 3), or (iv) a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.: 1, 2 and/or 3, wherein said peptide has a length of up to 100 amino acids and is optionally fused to a heterologous peptide or polypeptide.

According to some embodiments, the γ-Netrin-binding peptide, in particular the Netrin1-binding peptide, comprises any one of SEQ ID NO.: 7, SEQ ID NO.: 8, SEQ ID NO.: 9, SEQ ID NO.: 10, SEQ ID NO.: 11, SEQ ID NO.: 12, SEQ ID NO.: 13, SEQ ID NO.: 14 or SEQ ID NO.: 15 or a corresponding fragment of another species. Preferably, the peptide comprises any one of SEQ ID NO.: 10, SEQ ID NO.: 11, SEQ ID NO.: 12, SEQ ID NO.: 13, SEQ ID NO.: 14, and SEQ ID NO.: 15 or a corresponding fragment of another species, more preferably SEQ ID NO.: 15 or a corresponding fragment of another species.

In some preferred embodiments, the γ-Netrin-binding peptide, in particular the Netrin1-binding peptide, comprises a sequence which has a length of 22 amino acids. This 22-amino-acid (22aa) sequence may for example be SEQ ID NO.:16, or it may be SEQ ID NO.: 14, or it may be SEQ ID NO.: 17, or it may be a variant of any of these sequences, e.g. a corresponding fragment from another species with an additional amino acid residue, preferably glycine (Gly) at the N-terminus. Variants of SEQ ID NO.: 16, 14, 17 have a sequence identity of at least 70% to SEQ ID NO.: 16, 14 and/or 17. In further embodiments, the level of sequence identity may be at least 90%, or even at least 95% to SEQ ID NO.: 16, 14 and/or 17. In particular, a variant of SEQ ID NO.: 16, 14, 17 may differ in 1, 2, 3, 4, 5, 6 or 7 amino acids from SEQ ID NO.: 16, SEQ ID NO.: 14 and/or SEQ ID NO.: 17, provided they retain the γ-Netrin-binding activity.

Also encompassed by the invention is any variant of any one of SEQ ID NOs.: 7-15 or a corresponding fragment of another species having a sequence identity of at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% thereto, as long as the binding activity to γ-Netrins, preferably to Netrin1, is maintained. In some preferred embodiments, the level of sequence identity is at least 70%. In further preferred embodiments, the level of sequence identity is at least 90%.

In some embodiments, the γ-Netrin-binding peptide, in particular the Netrin1-binding peptide, according to the invention is fused to a heterologous peptide or polypeptide. A “heterologous peptide or polypeptide” in the context of the invention is any peptide with a length of at least 4 amino acids, which originates from another species as compared to the γ-Netrin-binding peptide or which is an artificial, i.e. non-naturally occurring peptide or polypeptide. Examples of such heterologous (poly)peptides are protein tags, in particular epitope tags such as the Myc-tag and the HA-tag, affinity tags such as FLAG, poly(His), chitin binding protein (CBP), maltose binding protein (MBP) and glutathione-S-transferase (GST), enzymes such as alkaline phosphatase, luciferase, horseradish peroxidase and β-galactosidase, or fluorescent proteins such as GFP, RFP and the like. Other examples are sequences that direct peptides attached thereto to specific locations inside cells or to the extracellular space (signal sequences); specific examples of such sequences are represented by SEQ ID NOs.: 70 and 71.

Further preferred examples of heterologous (poly)peptides are immunoglobulins (Ig) or functional fragments of immunoglobulins, such as Fv, scFv, Fab, Fab′, F(ab′)2, Fc, diabodies, minibodies, domain antibodies (dAb), camelid antibodies, nanobodies (VHH), disulfide stabilized Fv fragments (dsFv) and CDR-comprising peptides. The immunoglobulins or fragments thereof may be of any isotype, e.g. of the IgA-, IgD-, IgE, IgG or IgM-type. A functional structure analogous to the isotype G of immunoglobulins (IgG) is preferred. Among these, immunoglobulins or fragments thereof of the IgG1-, IgG2-, IgG3-, or IgG4-type are preferred.

In particularly preferred embodiments, the heterologous peptide or polypeptide is an Ig Fc fragment, for example an Fc fragment from mouse, rat, chicken, rabbit or human, with human Ig Fc fragments being preferred. Still more preferably, the human Ig Fc fragment is a human IgG Fc fragment, e.g. a human IgG1, IgG2, IgG3, or IgG4 Fc fragment.

The heterologous (poly)peptide may be conjugated to the γ-Netrin-binding peptide directly or via a spacer of suitable length. Suitable spacers are e.g. heterologous peptide linkers having a length of from 10 to 50, preferably from 10 to 30 amino acid residues. It is further preferred for the peptide linkers to be flexible linkers without a secondary structure. For example, suitable peptide linkers consist of at least 80% or at least 90%, preferably at least 95% or completely of glycine and/or serine residues. Particularly suitable are peptide linkers which contain a plurality of sequences SGGGG.

In some preferred embodiments of the invention, the inventive γ-Netrin-binding peptides, particularly the Netrin1-binding peptides, are competitive with Netrin receptors and can even release a γ-Netrin, e.g. human Netrin1 or zebrafish Netrin1a or 1 b, bound to a Netrin receptor.

In a further aspect, the invention relates to a γ-Netrin-binding peptide as defined herein for use in medicine. A “use in medicine” in the context of the invention may be a use in therapy and/or a use in diagnostics. Preferably, the γ-Netrin-binding peptide, in particular the Netrin1-binding peptide, according to the invention is for use in human medicine, but it may also be used for veterinary purposes.

In particular, a γ-Netrin-binding peptide comprising (i) the sequence EVMPTLDMALFDWTDYEDLKP (SEQ ID NO.:1), or (ii) the sequence DVAPTFNMALFDWTDYEDMRP (SEQ ID NO.:2), or (iii) the sequence EVMPTLDMTLFDWTDYEDMKP (SEQ ID NO.:3), or (iv) a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.:1, 2 and/or 3, which is optionally fused to a heterologous peptide or polypeptide, is for use in the prevention or treatment of a condition associated with, accompanied by or mediated by pathologic, particularly increased, γ-Netrin expression or activity. Preferably, the γ-Netrin is Netrin1.

A “pathologic expression or activity” of γ-Netrin, in particular Netrin1, as referred to herein is generally meant to encompass all situations, in which the ratio of expression and/or activity between Draxin and γ-Netrin and/or between γ-Netrin and at least one Netrin receptor is abnormal.

In particular, a “pathologic expression” means any level of expression, either on the DNA or the protein level, that differs from that of a normal healthy subject. Preferably, a pathologic γ-Netrin expression may be or may lead to a decreased γ-Netrin protein level or, more preferably, an increased γ-Netrin protein level, particularly an increased Netrin1 protein level, in a given (extracellular) environment of an organism. In accordance with preferred embodiments of the invention, the presence of an increased γ-Netrin level, particularly protein level, more particularly Netrin1 protein level, is defined as lying between 1 and 2 standard deviations above the average of healthy adults. Similarly, the presence of a decreased γ-Netrin protein level is defined as lying between 1 and 2 standard deviations below the average of healthy adults. For example, the γ-Netrin level in patient samples may be increased or decreased by a factor of at least 1,3 as compared to control samples from healthy adults.

A “pathologic activity” as referred to herein means in particular any activity of a protein that is abnormal. Preferably, a pathologic γ-Netrin activity may be a decreased or increased activity, e.g. binding to Netrin receptors with a lower or higher affinity. In accordance with preferred embodiments of the invention, the presence of an increased γ-Netrin activity, particularly Netrin1 activity, is defined as lying between 1 and 2 standard deviations above the average of healthy adults. Similarly, the presence of a decreased γ-Netrin activity is defined as lying between 1 and 2 standard deviations below the average of healthy adults. For example, the γ-Netrin activity in patient samples may be increased or decreased by a factor of at least 1,3 as compared to control samples from healthy adults.

A control group of “healthy adults” according to the invention can be determined by the skilled person with routine experimentation. For example, “healthy adults” for use as a possible control group herein may be healthy persons between 25 and 65 years of age, having a body mass index of 20-25, which may be sex-matched, if applicable.

The γ-Netrin-binding peptide for use as defined above may e.g. be a full-length Draxin from any vertebrate species, in particular human Draxin, rat Draxin, mouse Draxin, zebrafish Draxin A or zebrafish Draxin B. It may also be a variant of a full-length Draxin protein, characterized in that the variant differs in at least one, at least five, at least ten, at least 20 or even more amino acids from the complete sequence of the respective full-length Draxin protein, provided it retains the γ-Netrin-, in particular the Netrin1-binding activity. In preferred embodiments, the peptide is however significantly shorter as compared to full-length Draxin. For example, the peptide may have a length of up to 328, up to 324, up to 320, up to 300, up to 250, up to 200, up to 150, up to 100, up to 75 or up to 50 amino acids. In some preferred embodiments, the peptide has a length of up to 50 amino acids, e.g. 21 amino acids or 22 amino acids and is derived from zebrafish DraxinA. The peptide may or may not be fused to a heterologous peptide or polypeptide. Suitable peptides or polypeptides for fusion with the γ-Netrin-binding peptide are those described herein above, preferably protein tags, immunoglobulins (Ig) or functional fragments of immunoglobulins, wherein Ig Fc fragments, particularly human Ig Fc fragments are preferred. Still more preferably, the human Ig Fc fragment is a human IgG Fc fragment, e.g. a human IgG1, IgG2, IgG3, or IgG4 Fc fragment.

In some embodiments of the invention, the condition associated with, accompanied by or mediated by pathologic, particularly increased, γ-Netrin, preferably Netrin1 expression or activity is a hyperproliferative disease. A hyperproliferative disease according to the invention may be a tumor disease, a premalignant, non-neoplastic or non-malignant hyperproliferative disorder. In particular, the condition is a tumor disease.

An exemplary hyperproliferative disease which may be prevented or treated using the γ-Netrin-binding peptides, particularly Netrin1-binding peptides according to the invention is neoplastic lesions found in human inflammatory bowel disease (IBD).

Exemplary tumor diseases, which may be prevented or treated using the γ-Netrin-binding peptides, particularly Netrin1-binding peptides according to the invention, are selected from the group consisting of breast cancer, renal cancer, liver cancer, prostate cancer, colorectal cancer, lung cancer, neuroblastoma, meningioma of the brain, pituitary adenoma, glioma, glioblastoma, acute myeloid leukemia, sarcoma, melanoma, ovarian adenocarcinoma, renal adenocarcinoma, uterus adenocarcinoma, stomac adenocarcinoma, kidney adenocarcinoma and rectal adenocarcinoma, pancreatic cancer, inflammation driven cancers, particularly colorectal cancer, colorectal cancer associated with IBD, colorectal cancer associated with ulcerative colitis, colorectal cancer associated with Crohn's disease, and tumors derived from inflammatory bowel disease, including metastatic and particularly aggressive forms of these tumor diseases.

According to one embodiment, the tumor disease is neuroblastoma.

Preferably, the tumor disease is selected from the group consisting of pancreatic cancer, colorectal cancer, breast cancer, particularly metastatic breast cancer, and lung cancer, particularly non-small cell lung cancer.

In other embodiments of the invention, the condition associated with, accompanied by or mediated by pathologic γ-Netrin, particularly Netrin1 expression or activity is a cardiovascular disease, in particular atherosclerosis.

In still other embodiments of the invention, the condition associated with, accompanied by or mediated by pathologic γ-Netrin, particularly Netrin1 expression or activity is a neurological disorder, e.g. spinal cord injury.

The γ-Netrin-binding peptide, particularly Netrin1-binding peptide for use according to the invention may, according to a further aspect of the invention, also be present as an active agent in a pharmaceutical composition together with at least one pharmaceutically acceptable carrier. The present invention thus relates to a pharmaceutical composition comprising a γ-Netrin, particularly Netrin1-binding peptide or a salt or solvate thereof and at least one pharmaceutically acceptable carrier. Optionally, the pharmaceutical composition further comprises pharmaceutically acceptable excipients and/or adjuvants. Concentrations of these carriers, excipients and/or adjuvants, if used, are in a range that is physiologically acceptable.

A “carrier” as used herein must be physiologically acceptable and retain the therapeutic properties of the substance with which it is administered. Standard acceptable pharmaceutical carriers and their formulations are known to the skilled person. The carriers used will differ according to the administration route. Examples are magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatine, tragacanth, methylcellulose, sodium carboxymethylcellulose, a low melting-point wax, cocoa butter, water, alcohols, polyols, glycerol, vegetable oils, buffers etc.

As exemplary excipients, disintegrators, binders, fillers, and lubricants may be mentioned. Examples of disintegrators include agar-agar, algins, calcium carbonate, cellulose, colloid silicon dioxide, gums, magnesium aluminium silicate, methylcellulose, and starch. Examples of binders include microcrystalline cellulose, hydroxymethyl cellulose, hydroxypropylcellulose, and polyvinylpyrrolidone. Examples of fillers include calcium carbonate, calcium phosphate, tribasic calcium sulfate, calcium carboxymethylcellulose, cellulose, dextrin, dextrose, fructose, lactitol, lactose, magnesium carbonate, magnesium oxide, maltitol, maltodextrins, maltose, sorbitol, starch, sucrose, sugar, and xylitol. Examples of lubricants include agar, ethyl oleate, ethyl laureate, glycerin, glyceryl palmitostearate, hydrogenated vegetable oil, magnesium oxide, stearates, mannitol, poloxamer, glycols, sodium benzoate, sodium lauryl sulfate, sodium stearyl, sorbitol, and talc.

Usual stabilizers, preservatives, wetting and emulsifying agents, consistency-improving agents, flavour-improving agents, salts for varying the osmotic pressure, buffer substances, solubilizers, diluents, emollients, colorants and masking agents and antioxidants come into consideration as pharmaceutical adjuvants.

In yet a further aspect of the invention, the γ-Netrin-binding peptide, particularly Netrin1-binding peptide for use according to the invention may be widely combined with other therapeutically active agents, especially to achieve a synergistic effect in therapy and/or to be able to reduce the dosage of one or more active ingredients. Thus, the peptide is, in some embodiments, for use together with at least one additional therapeutically active agent, wherein the additional therapeutically active agent is particularly selected from the group consisting of decoy Netrin receptors, cytostatic agents, cytotoxic agents, statins, antihyperlipidemic agents, anti-coagulant agents, kinase inhibitors and angiogenesis modulators. Especially preferred therapeutic agents for combination with the γ-Netrin-binding peptide are cytostatic agents and cytotoxic agents.

A combination of a γ-Netrin-binding peptide, particularly a Netrin1-binding peptide, with at least one additional therapeutically active agent can be applied either by separate administration of the active ingredients to the patient or in the form of combination products in which a plurality of active ingredients are present in one pharmaceutical preparation. When the active ingredients are administered by separate administration of the active ingredients, this can be done simultaneously or consecutively.

The peptides according to the invention, either in an isolated form or as a pharmaceutical composition will typically be administered to a subject in need thereof, in particular a human subject. They will typically also be administered in a therapeutically effective amount, i.e. in an amount sufficient to achieve the desired effect. For example, one desired effect to be achieved by administration of γ-Netrin-binding peptides or anti-γ-Netrin antibodies or fragments or derivatives described herein may be to block, inhibit and/or neutralize one or more biological function of γ-Netrins, in particular of Netrin1, such as the binding to one or more Netrin receptor(s).

Administration of suitable compositions may be effected in different ways, e.g., by intravenous, intraperitoneal, subcutaneous, intramuscular, topical, oral, intradermal, intranasal or intrabronchial administration. Administration may also be conducted directly at the target site.

The necessary amount to be effective depends on a number of factors, such as the choice of the specific compound, the intended use, the administration route and the clinical condition of the patient. An appropriate “effective” amount in any individual case may be determined by a skilled person in the art, e.g. a skilled physician, using routine experimentation. An exemplary “therapeutically effective amount” of a peptide according to the invention may be about 0.01 mg to 50 mg/dose, preferably 0.1 mg to 10 mg/dose.

In yet a further aspect, the invention relates to an antibody or fragment or derivative thereof directed against Draxin, which inhibits binding of Draxin to γ-Netrins, in particular to Netrin1.

In yet a further aspect, the invention relates to an antibody or fragment or derivative thereof directed against Netrin1, which inhibits binding of at least one γ-Netrin, in particular of Netrin1, to at least one Netrin receptor, and which is directed against an epitope between amino acids 285-451 of human Netrin1. In particular, the antibody inhibits binding of at least one γ-Netrin, preferably Netrin1 to at least one of DCC (Deleted in colorectal cancer), DSCAM, DSCAM-L1, PTPRF (protein tyrosine phosphatase, receptor-type F), NEO (Neogenin), ADORA2B (adenosine A2B), Nope (Neighbor Of Punc Eli), and members of the UNC5H family (Uncoordinated-5 homologues; UNC5H1, UNC5H2, UNC5H3, UNC5H4).

The term “antibody” as used herein particularly refers to molecules comprising at least one immunoglobulin heavy chain and at least one immunoglobulin light chain. Each heavy and light chain may comprise a variable and a constant domain. The antigen-binding site may be formed from the variable domains of a heavy and a light chain. A variable region (also referred to as variable domain) comprises complementarity determining regions (CDRs), e.g. a CDR1, a CDR2 and a CDR3 region, and framework regions (FRs) flanking the CDRs. The term “complementarity determining region” is readily understood by the skilled person (see, for example, Harlow and Lane (eds.), Antibodies: A Laboratory Manual, CSHL Press, Cold Spring Harbor, N.Y., 1988; incorporated herein by reference in its entirety) and refers to the stretches of amino acids within the variable domain of an antibody that primarily make contact with the antigen and determine antibody specificity. This region is also known as the hypervariable region.

The term “(functional) antibody fragment or derivative thereof” as used herein encompasses fragments of antibodies, especially of human or humanized antibodies, such as portions of the above-mentioned antibodies which comprise at least one antigen-binding site. Examples of antibody fragments according to the invention include Fab fragments, Fab′ fragments, F(ab′)₂ fragments, Fv fragments, diabodies or single chain antibody molecules and other fragments as long as they exhibit the desired capability of binding to their target antigen, e.g. Draxin or at least one γ-Netrin. As exemplary single chain antibody molecules, scFv and nanobodies (VHH) are mentioned.

In case of antibodies or fragments or derivatives thereof directed against Draxin, only those antibodies, fragments and derivatives which inhibit the binding of Draxin to at least one γ-Netrin, preferably to Netrin1 are encompassed by the invention.

Likewise, in case of antibodies or fragments or derivatives thereof directed against γ-Netrin(s), only those antibodies, fragments and derivatives which inhibit the binding of at least one γ-Netrin, preferably Netrin1, to at least one Netrin receptor are encompassed by the invention.

Such inhibition can be determined by the skilled person via routine experimentation, e.g. via binding assays.

The term “bind” or “binding” of e.g. an antibody as used herein means an at least temporary interaction or association with or to e.g. a target antigen, e.g. Draxin or a γ-Netrin, comprising fragments thereof containing an epitope.

In preferred embodiments, the antibody directed against Draxin (i.e. the anti-Draxin antibody) according to the invention binds to an epitope on Draxin, which is located between amino acid residues 209-284, preferably between amino acid residues 226-257, of a zebrafish Draxin, preferably zebrafish DraxinA. More preferably, the antibody binds to an epitope between amino acids 232-252 of zebrafish DraxinA.

In further preferred embodiments, the anti-Draxin antibody according to the invention binds to an epitope on Draxin, which is located in a region of human Draxin corresponding to amino acid residues 209-284, preferably between amino acid residues 226-257, of zebrafish Draxin, preferably zebrafish DraxinA. More preferably, the antibody binds to an epitope between amino acids 222-243 of human Draxin.

In preferred embodiments, the antibody directed against γ-Netrin (i.e. the anti-γ-Netrin antibody) according to the invention binds to an epitope on Netrin1, which is located between amino acid residues 285-451 (i.e. EGF1-3), preferably between amino acid residues 341-451 (i.e. EGF2-3), of human Netrin1 or a corresponding epitope in another γ-Netrin and/or another species, preferably to an epitope located in the EGF1-3 domains, preferably the EGF2-3 domains of zebrafish Netrin1a and/or Netrin1b. More preferably, the antibody binds to an epitope between amino acids 404-451 (i.e. EGF3) of a human γ-Netrin, preferably human Netrin1 or to an epitope located in the EGF3 domain of a zebrafish γ-Netrin, preferably zebrafish Netrin1a or Netrin1 b.

The antibody or fragment or derivative thereof according to the invention may be derived from any antibody-producing animal species. Preferably, it is a mouse, rat or human antibody or functional antibody fragment or antibody derivative. The antibody may be a polyclonal antibody, a monoclonal antibody, a chimeric antibody, and/or a recombinant antibody. Monoclonal antibodies, in particular human or humanized monoclonal antibodies are preferred.

A monoclonal antibody (also referred to as mAB) is a single molecular species of antibody and is usually produced by creating hybrid antibody-forming cells from a fusion of nonsecreting myeloma cells with immune spleen cells. Polyclonal antibodies, by contrast, are produced by injecting an animal (such as a rodent, rabbit or goat) with an antigen, and extracting serum from the animal. A chimeric antibody is an antibody in which the variable domain of e.g. a murine antibody is combined with the constant region of a human antibody. Recombinant antibodies are obtained via genetic engineering without having to inject animals. Human antibodies according to the invention may be prepared using transgenic mice or by phage display; these methods are well known in the art.

In yet a further aspect, the antibodies or fragments or derivatives thereof according to the invention are for use in medicine (as defined above), particularly human medicine. For example, they may be used in diagnostics to determine qualitatively or quantitatively their respective antigens; they may also be used as a diagnostic agent for diseases with pathologic, in particular increased target expression.

In certain embodiments of yet a further aspect, the anti-γ-Netrin antibody (including its functional fragments and derivatives as defined herein) is for use in the prevention or treatment of a condition associated with, accompanied by or mediated by pathologic, particularly increased, γ-Netrin, particularly Netrin1 expression or activity, as defined herein above. This condition is preferably a hyperproliferative disease, in particular a tumor disease.

Exemplary tumor diseases, which may be prevented or treated using the anti-γ-Netrin antibody, preferably anti-Netrin1-antibody, or fragments or derivatives thereof according to the invention, are selected from the group consisting of breast cancer, renal cancer, liver cancer, prostate cancer, colorectal cancer, lung cancer, neuroblastoma, meningioma of the brain, pituitary adenoma, glioma, glioblastoma, acute myeloid leukemia, sarcoma, melanoma, ovarian adenocarcinoma, renal adenocarcinoma, uterus adenocarcinoma, stomac adenocarcinoma, kidney adenocarcinoma and rectal adenocarcinoma, pancreatic cancer, inflammation driven cancers, particularly colorectal cancer, colorectal cancer associated with IBD, colorectal cancer associated with ulcerative colitis, colorectal cancer associated with Crohn's disease, and tumors derived from inflammatory bowel disease, including metastatic and particularly aggressive forms of these tumor diseases.

Preferably, the tumor disease is selected from the group consisting of pancreatic cancer, colorectal cancer, breast cancer, particularly metastatic breast cancer, and lung cancer, particularly non-small cell lung cancer. In another embodiment, the tumor disease is neuroblastoma.

The anti-γ-Netrin antibody, preferably anti-Netrin1-antibody or fragment or derivative thereof may also be present in the form of a pharmaceutical composition as defined above, and may also be used in combination with further pharmaceutically active agents, particularly one or more agent selected from the group consisting of decoy Netrin receptors, cytostatic agents, cytotoxic agents, statins, antihyperlipidemic agents, anti-coagulant agents, kinase inhibitors and angiogenesis modulators. Especially preferred therapeutic agents for combination with the anti-γ-Netrin-antibody are cytostatic agents and cytotoxic agents.

In certain embodiments of yet a further aspect, the anti-Draxin antibody (including its functional fragments and derivatives as defined herein) is for use in the prevention or treatment of a condition associated with, accompanied by or mediated by pathologic, particularly decreased, γ-Netrin, particularly Netrin1 expression or activity, as defined herein above. Likewise, the anti-Draxin antibody (including its functional fragments and derivatives as defined herein) may be used in the prevention or treatment of a condition associated with, accompanied by or mediated by pathologic, particularly increased, Draxin expression or activity.

This condition may be a cardiovascular disorder, in particular a cardiovascular disorder which can be prevented or treated by increasing γ-Netrin, in particular Netrin1 expression and/or activity. Exemplary cardiovascular disorders are selected from ischemia/reperfusion (I/R) injury, e.g. renal I/R injury, I/R injury of cardiac tissue; myocardial infarction, particularly infarcts resulting from I/R injury; mitochondrial damage; neointimal formation and restenosis; vascular injury or vascular dysfunction; vascular smooth muscle cell migration and proliferation; apoptosis of endothelial progenitor cells, procure induced restenosis; and hypertension.

A “pathologic expression or activity” of Draxin is generally meant to encompass all situations, in which the ratio of expression and/or activity between Draxin and any γ-Netrin is abnormal. Particularly, a pathologic Draxin expression may be or may lead to a decreased Draxin protein level or, preferably, an increased Draxin protein level in a given (extracellular) environment of an organism. A pathologic Draxin activity may be an decreased or increased activity, e.g. binding to a γ-Netrin, particularly Netrin1 with a lower or higher affinity.

In yet a further aspect, the present invention relates to peptides which bind to Draxin, in particular to human Draxin and/or zebrafish DraxinA and/or zebrafish DraxinB, with a high specificity and a high affinity.

Accordingly, the invention provides a Draxin-binding peptide comprising (i) at least 20 consecutive amino acids from the sequence KACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCANGYQQSRSPIAPC IKIPIAPP (SEQ ID NO.: 51) or (ii) a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.:51, wherein said peptide has a length of up to 580 amino acids and is optionally fused to a heterologous peptide or polypeptide.

In particular, the Draxin-binding peptide comprises at least 20, at least 30, at least 40, at least 50 consecutive amino acids from SEQ ID NO.:51 or the complete SEQ ID NO.: 51 or a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.:51.

In some embodiments, the peptide has a length of up to 580 amino acids, up to 500 amino acids, up to 400 amino acids, up to 300 amino acids, up to 250 amino acids, up to 200 amino acids, up to 100 amino acids, up to 75 amino acids, or up to 60 amino acids. Fragments comprising up to 60 amino acids are preferred according to some embodiments.

In some embodiments, the peptide with a length of up to 580 amino acids comprises SEQ ID NO.: 51 or a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.: 51 and is free of any further heterologous peptides.

In other embodiments, the peptide with a length of up to 580 amino acids comprises SEQ ID NO.: 51 or a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.: 51 and is fused to a heterologous peptide or polypeptide as defined above, preferably protein tags, immunoglobulins (Ig) or functional fragments of immunoglobulins, wherein Ig Fc fragments, particularly human Ig Fc fragments are preferred. Still more preferably, the human Ig Fc fragment is a human IgG Fc fragment, e.g. a human IgG1, IgG2, IgG3, or IgG4 Fc fragment.

In some embodiments, the peptide may also be fused to a heterologous signal sequence, e.g. a human Netrin1-fragment comprising SEQ ID NO.: 51 may be fused to a zebrafish signal sequence (comprising e.g. SEQ ID NO.: 70 or 71), as shown in SEQ ID NOs.: 68 and 69.

In a further aspect, the invention relates to a Draxin-binding peptide as defined herein for use in medicine, particularly human medicine.

In certain embodiments of yet a further aspect, the Draxin-binding peptide described herein is for use in the prevention or treatment of a condition associated with, accompanied by or mediated by pathologic, particularly decreased, γ-Netrin, in particular Netrin1 expression or activity, as defined herein above. Likewise the Draxin-binding peptide may be used in the prevention or treatment of a condition associated with, accompanied by or mediated by pathologic, particularly increased, Draxin expression or activity.

This condition may be a cardiovascular disorder, in particular a cardiovascular disorder which can be prevented or treated by increasing γ-Netrin, in particular Netrin1 expression and/or activity. Exemplary cardiovascular disorders are selected from ischemia/reperfusion (1/R) injury, e.g. renal I/R injury, I/R injury of cardiac tissue; myocardial infarction, particularly infarcts resulting from I/R injury; mitochondrial damage; neointimal formation and restenosis; vascular injury or vascular dysfunction; vascular smooth muscle cell migration and proliferation; apoptosis of endothelial progenitor cells, procure induced restenosis; and hypertension.

The Draxin-binding peptide for use according to the invention may, according to a further aspect of the invention, also be present as an active agent in a pharmaceutical composition together with at least one pharmaceutically acceptable carrier. The present invention thus relates to a pharmaceutical composition comprising an Draxin-binding peptide thereof and at least one pharmaceutically acceptable carrier and, optionally, pharmaceutically acceptable excipients and/or adjuvants.

Still a further aspect of the present invention is a method for the treatment of a condition associated with, accompanied by or mediated by pathologic, particularly increased or decreased γ-Netrin, in particular Netrin1 expression or activity, comprising administering a γ-Netrin-binding peptide, a Draxin-binding peptide, an anti-γ-Netrin antibody and/or an anti-Draxin antibody as described herein above to a subject, particularly a human subject in need thereof. In particular, this subject suffers from a hyperproliferative disorder as defined above.

The invention also refers to a nucleic acid molecule, preferably an isolated nucleic acid molecule, encoding a γ-Netrin-binding peptide, particularly the Netrin1-binding peptide, the Draxin-binding peptide, and the antibodies or fragments or derivatives thereof directed against γ-Netrin, in particular Netrin1, and/or Draxin as described above. The term “nucleic acid molecule” encompasses a natural DNA or RNA or a recombinantly or synthetically produced DNA, RNA or LNA or a recombinantly produced chimeric nucleic acid molecule comprising any of these nucleic acids either alone or in combination. For example, the nucleic acid may be cDNA or genomic DNA corresponding to an entire gene or a substantial portion thereof or to fragments and derivatives thereof. The nucleotide sequence may correspond to the naturally occurring nucleotide sequence or may contain single or multiple nucleotide substitutions, deletions or additions. The nucleic acid may also be fused to another nucleic acid. The nucleic acid molecule of the invention may be in operative linkage to an expression control sequence, i.e. to a sequence which is necessary to effect the expression of coding nucleic acid sequences. Such expression control sequences may include promoters, enhancers, ribosomal binding sites and/or transcription termination sequences. Specific examples of suitable expression control sequences are known in the art.

The nucleic acid molecule of the invention may be located on a vector which may additionally contain a replication origin and/or a selection marker gene. Examples of vectors are plasmids, cosmids, phages, viruses etc.

Further, the invention refers to a recombinant cell, which comprises the nucleic acid molecule as described above. The nucleic acid molecule may be introduced into the recombinant cell by transformation, transfection or transduction according to any method known in the art. The recombinant cell may e.g. be a prokaryotic or eukaryotic cell. Preferably, the cell is a mammalian cell, e.g. a hamster, rabbit, or human cell. Preferably, the cell is a human cell.

The γ-Netrin-binding peptide, the Draxin-binding peptide, and the antibodies or fragments or derivatives thereof directed against γ-Netrin and/or Draxin of the invention may be prepared by a method, wherein the cell as described above is cultured under conditions which allow expression of the antibody encoding nucleic acid molecule. The antibody may be collected from the cultured cell or the culture supernatant. Preferably, the antibody is prepared from a mammalian, particularly from a human cell.

Also encompassed by the invention are salts and solvates, preferably pharmaceutically acceptable salts and solvates of the disclosed peptides, in particular of the γ-Netrin-, preferably Netrin1-binding peptides and the Draxin-binding peptides.

Pharmaceutically acceptable salts may include, but are not limited to, acid addition salts and basic salts. Examples of acid addition salts include chloride, sulfate, hydrogen sulfate, (hydrogen) phosphate, acetate, citrate, tosylate or mesylate salts. Examples of basic salts include salts with inorganic cations, e.g. alkaline or alkaline earth metal salts such as sodium, potassium, magnesium or calcium salts and salts with organic cations such as amine salts.

A “solvate” is a complex of a peptide of the invention or a salt thereof with solvent molecules, e.g. organic solvent molecules and/or water.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1. Draxin directly binds Netrin1.

A) AVEXIS screen results showing that the DraxinA prey protein, in addition to the positive control bait Matn4 (+), only binds to Netrin1a in a protein library consisting of 171 bait proteins. B) Interaction network of the zebrafish Netrin1 and Draxin paralogs. Consecutive screen results show that the interaction is also conserved for the paralogs.

FIG. 2. The binding of Draxin to Netrin1 is conserved for the human homologs and detectable across human and zebrafish proteins.

Heatmap showing results from the AVEXIS assay. The screen has been performed in both bait-prey orientations using pentameric prey proteins. Absorbance at 486 nm (A486 nm) has been measured after 1-hour incubation (black: A486 nm>0.1=binding, grey: A486 nm 0.08-0.1=weak binding, 2 repeats). B) Network view of the results.

FIG. 3. Draxin protein alignment using the Clustal W method.

The alignment of human, mouse, chick, and zebrafish DraxinA and DraxinB protein shows that the N-terminal half of the protein is poorly conserved. In the most C-terminal part of the protein a conserved 10-cysteine containing region can be found, which resembles the cysteine-rich region present in Dickkopf proteins.

FIG. 4. A conserved 21aa DraxinA derived peptide is sufficient for the binding to Netrin1a.

A) By using a set of truncated and deletion containing monomeric DraxinA preys the binding interface of DraxinA to Netrin1a has been mapped down to a 21 amino acid region (aa232-aa252). The protein fragments binding to Netrin1a are indicated in black, weak binding ones in grey, and non binding fragments are depicted in white. B) and C): Using a similar approach the DraxinA binding interface in Netrin1a has been narrowed down to the third EGF-domain containing region (aa401-aa458).

FIG. 5. Multiple species alignment of the Draxin derived 21 as peptide sequence.

The figure shows that the Netrin1 binding peptide is highly conserved in vertebrates.

FIG. 6. DraxinA inhibits the binding of Netrin1a to Netrin receptors.

A) Schematic representation of the AVEXIS based competition assay. B) Purified full-length Draxin inhibits the binding of of the Netrin1a bait to the DCC-prey proteins. C) This effect can also be seen for the Unc5b and Neo1 (D) netrin receptors. Equal amounts of BSA (D) are not able to inhibit the binding between Netrin and Neo1. E) Draxin is not able to interfere with the binding of RGMc to Neo1. RGMc is another known Neo1 ligand. (% binding: binding with inhibitor/binding without inhibitor X100%, error bars indicate mean±s.d.; n=4)

FIG. 7. A 21 amino acid fragment of Draxin is sufficient to outcompete Netrin/Netrin receptor interactions.

A) Full-length DraxinA-hFc and the 21aa peptide fused to the hFc region are able to outcompete DCC for binding to Netrin1a. In contrast the hFc fusion of full-length DraxinA with a deletion of aa231-252 is not able to compete for binding. None of the 3 DraxinA-hFc fusion protein versions is able to block the binding between other known tested receptor-ligand pairs: (B) Cntn1a/Ptprz1b, (C) Vasn/Islr2, and (0) EphB4a/EphrinB2a. (% binding: binding with inhibitor/binding without inhibitor X100%, error bars show mean±s.d.; n=3)

FIG. 8. The binding of the 21aa DraxinA derived peptide is highly specific.

A) A pentameric DraxinA aa232-252-prey protein has been screened against a library consisting of 141 bait proteins. We only observed binding to the Netrin1a-bait. The positive control (+) corresponds to Matn4. B) The DraxinA aa232-252-bait has been screened against 191 pentameric prey proteins and only showed binding to the Netrin1a prey.

FIG. 9. Draxin outcompetes receptor bound Netrin1a.

A) Scheme of the experimental design. B) The inhibitory effect of DraxinA is not decreased by already preformed Netrin1a/DCC complexes.

FIG. 10: Protein alignment of the third EGF domain of Netrins.

Protein alignment of the third EGF domain of human Netrin1, Netrin3, Netrin4 and zebrafish Netrin1a and Netrin1b shows that this domain is highly conserved for the γ-chain netrins but not for Netrin4 which belongs to the laminin1β-chain derived netrins. In agreement with the protein alignment data our AVEXIS binding data indicate that only γ-chain derived Netrins can bind to Draxin.

FIG. 11: In vivo detection of the Draxin-Netrin1a interaction in zebrafish embryos.

A) Schematic illustration of the assay design. mRNAs encoding the indicated fluorophore tagged genes were injected into one-cell stage zebrafish embryos and imaged at sphere stage (4 hours post fertilization (hpf)). The imaging plane corresponded to a region approximately 15 μm beneath the enveloping layer of the embryos. B) Single section confocal images of the embryos. (Ba, Ba′, Ba″) embryos injected with 100 pg Draxin-super folder GFP (sfGFP) mRNA displayed uniform distribution of Draxin-sfGFP protein in the extracellular space. In contrast injection of 100 pg of Netrin1a-sfGFP mRNA (Bb, Bb′, Bb″) resulted in dense membrane associated speckles positive for Netrin1a-sfGFP protein. In (Ba) and (Bb) memRFP has been used to label the cell surface. Upon co-injections of 200 pg Draxin-sfGFP mRNA and 200 pg of Netrin1a-mCherry mRNA (Bc, Bc′, Bc″) Draxin-sfGFP and Netrin1a-mCherry proteins co-localize into membrane associated spots. (n=7; arrowheads point to examples of co-localization; scale bars correspond to 10 μm).

FIG. 12: In situ detection of the Draxin-Netrin1a interaction in zebrafish using an affinity probe.

A) Schematic illustration of the experiment. A Draxin_(aa209-284)-hFc-fusion protein was generated in HEK293-6E cells as a probe to detect endogenous netrins. Mildly fixed 48 hpf embryos were incubated with the affinity probe (Draxin_(aa209-284)-hFc) and the signal detection has been carried out by using a fluorophore tagged anti human IgG antibody.

B) Results of the in situ detection experiments. The signal from Draxin_(aa209-284)-hFc-fusion probe was detectable in the floor plate region in wild type (also abbreviated as wt) fish (Ba), and was not detectable in netrin1a and netrin1b double-knockdown embryos (Bb). The shh:GFP transgenic line has been used for the experiments to visualize floor plate cells. Arrowheads point to the Draxin_(aa209-284)-hFc derived signal. Scale bar in (Ba″), 20 μm; applies to all panels. (n>10)

FIG. 13: Heatmap depicting binding results between human DRAXIN and Netrin signaling system members.

AVEXIS was used to test pairwise binding events in both bait/prey orientations between human DRAXIN, the derived 21 amino acid peptide, human Netrin family members, and two representative Netrin receptors (DCC, UNC5B). The Matn4-bait served as an internal prey-protein control and was used for normalization of the A486 nm values; conditioned medium (CM) serves as negative control; n=3.

FIG. 14: Surface Plasmon Resonance Analysis of Draxin Binding.

Binding of Draxin to immobilised recombinant human Netrin1, UNC5B, and DCC was monitored using Surface Plasmon Resonance experiments on a Biacore 3000 instrument. See text for details.

The invention will be further illustrated by the following examples.

EXAMPLES Methods AVEXIS Based Library Screen

To detect extracellular protein-protein interactions in a high-throughput manner we used the AVEXIS assay as described in Bushell et al. (Bushell et al., 2008) with minor modifications.

In brief: A zebrafish protein library enriched for mainly in neuronal tissues expressed secreted proteins and extracellular domains of cell surface proteins has been assembled. The library consists of prey and bait proteins. Preys are composed of the extracellular domain (ECD) of interest followed by a CD4 tag (rat Cd4d3+4) and a pentamerization domain derived from the rat Comp protein followed by β-lactamase. For the bait proteins the ECDs are fused to a CD4 tag and a biotinylation peptide. All proteins for the screen have been expressed by transient transfection of Human Embryonic Kidney (HEK293-6E) cells (Durocher et al., 2002) grown in Freestyle medium (Invitrogen) containing 1% FCS. Supernatants have been harvested 6 days post transfection. The bait proteins have been dialyzed against HBS (140 mM NaCl, 5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 10 mM HEPES, pH 7.4) to remove free biotin. The proteins in the supernatants have been quantified and normalized.

For the AVEXIS screen the supernatant dilution factors have been adjusted to values allowing faithful detection of the interaction between Vasn (Slit-like2) and Islr2 (Söllner and Wright, 2009) with determined KD of 12 μM and a very short half-life (t½ 0.16 s) (in preparation). The bait proteins have been immobilized on streptavidin coated 96 well microtiter plates (one bait/well) and incubated for 1 hour at room temperature. After 3 wash steps using HBS as wash buffer the baits have been probed by 50 μl of normalized prey proteins. After one-hour incubation the non-bound preys have been washed away by 2 washes with HBST (0.05% Tween) followed by two HBS washes. Then 50 μl of nitrocefin/well (0.1 mg/ml) has been added and incubated for 1 h at room temperature. Then the absorbance at 486 nm of each well has been measured using a μQuant spectrophotometer (BIO-TEK Instruments, INC), As a positive control for the prey proteins we used the Matn4 ECD as mono-biotinylated bait protein. Matn4 has been shown to bind to the coiled-coil pentamerization domain of Comp (Mann et al., 2004), which is present in all recombinant pentameric prey proteins of our AVEXIS library.

In the primary screen interactions were ‘called’ if the absorbance (at 486 nm) of a well after 1 h of incubation was ≧0.1 and 3 Sigma above the standard deviations of the mean of all wells. All interactions detected in the primary screen have been retested in a validation screen by using independently produced batches of proteins.

Domain Mapping Experiments

For the binding interface mapping experiments we used monomeric prey proteins. The concentration of the monomeric preys has been determined as described for the AVEXIS assay (β-lactamase enzymatic activity). After normalization, the monomeric preys have been screened against a set of proteins from the library to identify and remove promiscuous binders caused for example through improper domain boundary design. Both prey and bait orientations were tested in the domain mapping screen and two repeats for each orientation had been carried out.

Protein Purification

His-tagged full-length zebrafish Draxin protein (Draxin-CD4d3+4-6XHis) has been expressed in HEK293-6E cells and affinitiy purified from tissue culture supernatants using HisTrap HP columns (GE healthcare). The correct size of the purified protein has been checked on a protein gel.

AVEXIS Based Competition Assay

The procedure of the competition assay is based on the AVEXIS assay. Netrin1 receptors were used as prey proteins together with 6XHis-tagged purified Draxin (potential antagonist), and probed against Netrin1a bait proteins. The indicated concentrations of the potential inhibitors have been added together with prey proteins. For the competition tests with purified Draxin the concentration of the Netrin receptor prey proteins has been adjusted to an identical threshold binding concentration.

hFc Fusion Protein Normalization for the Competition Assay

We determined the concentration of ECD-hFc fusion proteins in tissue culture supernatants by ELISA, using the human IgG Fc fragment (Calbiochem) as a reference. Dilution series of the ECD-hFc containing supernatants have been incubated over night at 4° C. on 96 well Maxisorp plates (Nunc). After 3 PBS washes the plates were blocked with 0.5% BSA containing PBS (1 h). After additional 2 PBS washes and 1-hour incubation with an anti-human IgG (Fc specific) antibody fused to alkaline phosphatase (SIGMA) the plates have been washed 3 times with PBS. The detection has been carried out by addition of 50 μl/well of the AP substrate p-nitrophenyiphosphate (SouthernBiotech). The substrate turnover has been determined by measuring the absorbance at 405 nm.

In Vivo Binding Assay

To reveal whether the interaction between Draxin and Netrin1a is detectable in vivo, an mRNA overexpression assay was designed to visualize the localization of the two proteins in zebrafish embryos. Constructs of full-length zebrafish Draxin and Netrin1a fused with fluorescent proteins were generated by using the Gateway® cloning system (Life Technologies).

Before used in the in vivo detection assay, the coding sequences of the generated fusion proteins were cloned into AVEXIS plasmids, expressed as preys and tested for activity against bait proteins of the corresponding binding partner. Following constructs were selected for the in vivo binding test: Draxin and Netrin1a C-terminally fused to superfolder-GFP (Draxin-sfGFP, Ntn1a-sfGFP) and Netrin1a C-terminally fused with mCherry (Ntn1a-mCherry). The corresponding capped mRNAs were synthesized using the mMESSAGE mMACHINE SP6 or T7 Transcription Kit (Ambion) according to manufacturer instructions. For the injections, zebrafish embryos were dechorionated using 1 mg/ml Pronase (Roche, 11459643001) and then injected with 1 n1 mRNA into the cell center at one cell stage. Draxin-sfGFP or Netrin1a-sfGFP mRNAs were injected at 100 pg/embryo in combination with 10-15 pg/embryo of membrane-tagged RFP (mRFP) to label the cell membranes. The same amount of mRNA (100-200 pg/embryo) was injected in the Draxin-sgGFP and Netrin1a-mCherry coexpression experiments. The injected embryos have been cultured at 28.5° C. in agarose-coated dishes. At sphere stage (4 hpf), embryos were immobilized in 1% low-melting-point agarose in glass-bottom Petri dishes with the animal pole facing the coverslip. The imaging plane corresponded to a region approximately 15 μm beneath the enveloping layer of the embryos. Single plane confocal images of the embryos were taken using a Zeiss LSM 780 NLO microscope.

In Situ Detection of Draxin Binding Partners

A netrin binding fragment of Draxin (aa209-284) fused to the Fc region of human IgG (Draxin_(aa209-284)-hFc) has been expressed in HEK293-6E cells and used as an affinity probe to detect binding partners in zebrafish embryos. Draxin_(aa209-284)-hFc in situ staining has been done in whole mount wild type and netrin-1 knockdown zebrafish embryos. At 46 hpf wild type zebrafish embryos were dechorionated by for 2 hours at RT incubation in 0.1 mg/ml Pronase. At 48 hpf embryos were prefixed for 10 min at RT in 4% (w/v) paraformaldehyde (PFA) containing 1% Triton X-100 (v/v). After 3 washes (each 20 min) with PBS+1% Triton X-100 the embryos were blocked for 4 h at RT in PBS containing 0.2% BSA and 0.5% Triton X-100. Overnight incubation at 4° C. with HEK293-6E supernatant containing the Draxin-hFc fusion protein was followed by 3 short washes (10 min each) with PBST. Subsequently the embryos were post-fixed in 4% PFA (4 h at RT or overnight at 4° C.), rinsed shortly 3 times with PBST and incubated for 4 h at RT with an Alexa Fluor 568 goat anti human IgG antibody (Invitrogen, 1:250 dilution). After 3 washes (30 min each) in PBST the embryos were mounted in glycerol for visualization using a Zeiss LSM 510 microscope. A shorter version of Draxin containing the Netrin1a binding site had to be used because the full-length version of Draxin-hFc caused uniform background staining in zebrafish embryos probably by unspecific binding to glycosaminoglycans (GAGs) present on cell surfaces. A series of additional control ECD-hFc proteins have been tested, only Draxin_(a209-284)-hFc displayed binding to the extracellular space of the floor plate.

Knockdown of netrin1a and netrin1b in Zebrafish Embryos

Morpholino antisense oligonucleotides (Gene-Tools) have been used to generate zebrafish with reduced netrin protein expression levels. The following morpholino sequences have been used to knockdown ntn1a (5′-ATGATGGACTTACCGACACATTCGT-3′, SEQ ID NO.: 80) and ntn1b (5′-CGCACGTTACCAAAATCCTTATCAT-3′, SEQ ID NO.: 81). In previous studies both morpholinos had been shown to efficiently knockdown the corresponding genes (Kastenhuber et al., 2009; Suli et al.; 2006).

Surface Plasmon Resonance (SPR)

Surface Plasmon Resonance (SPR) experiments were performed on a Biacore 3000 (GE Healthcare) at 25° C. using a SA sensor chip in 0.01 M HEPES, pH 7.4, 0.15 M NaCl, 0.005% Surfactant P20 (HBS-P) running buffer at a flow rate of 30 μl/min. The instrument was used according to manufacturer's instructions.

Example 1 DraxinA Physically Interacts with Netrin1a

Using a protein-protein interaction screen assay, designed to identify direct interactions within a protein library consisting of secreted proteins and extracellular domains of cell surface proteins (Bushell et al., 2008), we carried out a large-scale screen involving more than 40,000 binding experiments. The library we used for the screen was strongly enriched for zebrafish proteins known to be expressed in the developing nervous system. During this screen we identified a novel interaction between two secreted proteins with known function in axon guidance, Netrin1a and DraxinA. In the primary screen a DraxinA prey protein has been tested for binding against a library consisting of 171 bait proteins, including a positive control. The DraxinA prey protein specifically bound to the Netrin1a bait (FIG. 1A) and did not bind to any additional proteins of the library. The interaction has been confirmed in both bait-prey orientations in a validation screen using new protein samples. Interestingly, both netrin1 and draxin are duplicated in zebrafish. In subsequent binding assays we were able to show that the Netrin1/Draxin interaction is also conserved for the paralogs Netrin1b and DraxinB (FIG. 1B).

The AVEXIS assay is able to detect very transient and weak interactions due to the avidity effect caused by the use of pentameric prey proteins. Hence, in order to test whether the interaction between Netrin1a and Draxin is transient or rather stable we used monomeric prey proteins and probed them against the corresponding binding partners. Using this approach, we confirmed the interaction between Netrin1a and Draxin suggesting that this interaction is based on strong binding between the two proteins.

Example 2 The Interaction Between Draxin and Netrin1 is Conserved for the Human Homologs

Next we asked the question whether the interaction between Netrin1 and Draxin is conserved. By using the corresponding human homologs NTN1 and DRAXIN we were able to show that the interaction is indeed conserved. In addition, we observed that zebrafish Netrin1a was able to bind to human Draxin and vice versa (FIG. 2). This strongly indicates that this newly identified interaction is conserved within vertebrate species underscoring the biological relevance of this interaction.

Example 3 The Netrin1 Binding Region of Draxin has been Mapped Down to a 21aa Motif

Next, we narrowed down the region in DraxinA required for binding to Netrin1a. Zebrafish DraxinA consists of 360 amino acids (aa). The first 23 as are part of the signal peptide. This sequence is followed by a poorly conserved N-terminal half of the protein (FIG. 3). In contrast, the C-terminal half of the protein is highly conserved and ends with a 10 cysteine-containing domain (aa285-aa360). In terms of cyteine spacing this domain is similar to domains present in the Wnt antagonist Dkk1 (Glinka et al., 1998).

To map down the Netrin1a binding region in DraxinA we generated a series of DraxinA truncations and deletions and tested them for binding against Netrin1a (FIG. 4). Using this approach we were able to narrow down the binding region to a 21aa DraxinA protein fragment. In addition, a full-length version of DraxinA lacking these 21aa completely lost the ability to bind to Netrin1a. Additional removal of 5aa from the N-terminal or C-terminal end of the 21aa stretch caused a dramatic reduction of the binding ability to Netrin1a. Interestingly, the Netrin-binding 21aa stretch (aa232-252) of DraxinA is highly conserved cross vertebrate species (FIG. 5). It is noteworthy that, this conserved 21aa region is also highly specific for Draxin and cannot be found in other proteins.

Example 4 Netrin1a Domain Mapping

Netrin1a is a multi-domain containing protein composed of 603 amino acids. It consists of a laminin N-terminal domain (LamNT) encoded by amino acid 44-282 followed by 3 laminin-type epidermal growth factor-like domains (aa284-450), and a C-terminal domain (C345C) encoded by amino acid 486 to 594. In order to map the DraxinA binding region in Netrin1a we generated a set of truncated Netrin1a fragments and probed them in the AVEXIS assay for binding against DraxinA. Using this approach we were able to narrow down the binding region to a fragment consisting of amino acid 401-458 (FIG. 4B). This fragment encodes the third laminin-type EGF domain. The third EGF-domain of Netrin1a is highly conserved in vertebrate Netrin1 homologs. For example, only a single amino acid exchange is present in this domain between zebrafish Netrin1a (CDCHPVGAAGKTCNQTTGQCPCKDGV TGITCNRCANGYQQSRSPIAPC; SEQ ID NO.: 64) and human Netrin1 (CDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPC; SEQ ID NO.: 65) proteins.

Interestingly, the third EGF domain of Netrin1 has recently been shown to be required for Netrin receptor binding (Finci et al., 2014; Xu et al., 2014). These findings offer a mechanistic explanation for our observed competition assay results.

Example 5 DraxinA is Able to Inhibit the Binding of Netrin1a to Netrin Receptors

By using an AVEXIS-based competition assay (FIG. 6 A) we tested whether the binding of DraxinA to Netrin1a has an influence on Netrins ability to bind to Netrin receptors. First we confirmed that we reliably detected the binding of Netrin1a to Netrin receptors of the DCC/Neo1 and Unc5 families with the AVEXIS method. We also tested whether Draxin is able to bind to the corresponding Netrin receptors. Contrary to previous findings (Ahmed et al., 2011), we were not able to detect direct binding between Draxin and any of the tested Netrin receptors using the AVEXIS platform. This has also been confirmed by a recent publication (Haddick et al. 2014). In the competition assay the extracellular domains (ECDs) of Netrin receptors have been used as prey proteins together with purified full length DraxinA and probed for binding against Netrin1a bait proteins. Using this strategy, we observed a DraxinA concentration dependent inhibition of the binding between Netrin1a and Netrin receptors (FIG. 6 B,C,D). The inhibition is specific for DraxinA. Furthermore, DraxinA (FIG. 6 E) is not able to block the binding of RGMc to Neo1, another reported ligand of the corresponding receptor (Bell et al., 2013).

Example 6 The 21Aa DraxinA Fragment Fused to the Human Fc Region of IgG is Sufficient to Block the Binding of Netrin1a to Netrin Receptors

Next we assayed whether the 21aa DraxinA fragment fused to the human Fc tag (Draxin_(aa232-252)-hFc) is sufficient to outcompete Netrin/Netrin receptor interactions.

We compared the effect of Draxin_(aa232-252)-hFc with full-length Draxin-hFc and a version of DraxinA where the 21aa Netrin1 binding motif has been deleted (DraxinAΔ_(aa231-252)-hFc) in the competition assay for their ability to interfere with the binding of Netrin1a to Dcc. The results show that Draxin_(aa232-252)-hFc has a similar efficiency in inhibiting the binding of Netrin1a to Dcc as the DraxinA full-length version (Draxin-hFc). The DraxinA version with the 21aa deletion (DraxinAΔ_(aa231-252)-hFc) is not able to compete for binding to Netrin1a (FIG. 7). In addition we used our set of different Draxin-hFc proteins to test whether they have an effect on other known interactions (Cntn1a/Ptprz1b, Vasn/Islr2, EphB4a/EphrinB2a). None of the 3 Draxin-hFc versions was able to inhibit any of the tested interactions (FIG. 7 B,C,D).

Taken together, our results show that the 21aa region is necessary for the competition and that the Draxin_(aa232-252)-hFc fusion protein is also sufficient to outcompete Netrin receptors for Netrin1a binding.

Example 7 The Binding of the 21Aa DraxinA Fragment to Netrin1a is Highly Specific

In order to determine the binding specificity of the 21aa DraxinA peptide we used the AVEXIS assay and screened the 21aa fragment as bait and prey against proteins from our library. In this screen against a set of 141 bait proteins the DraxinA_(aa232-252)-prey only bound to the Ntn1a-bait and to the positive control bait Matn4 (FIG. 8A). Similar results we obtained for the DraxinA_(aa232-252) bait protein, which only interacted with the Ntn1a-prey in a screen against a set of 191 different prey proteins (FIG. 8B). These findings indicate that the binding of this short 21aa peptide to Netrin1a is highly specific.

Example 8 DraxinA Outcompetes Receptor Bound Netrin 1a

Netrin1 binds with high affinity (K_(d)'s in the low nM range) to its receptors of the DCC- and Unc5-family (Leonardo et al., 1997). Hence, we asked the question whether already bound Netrin1a could be displaced from the receptors by DraxinA. In order to do so we carried out an AVEXIS based competition assay and tested three different settings (FIG. 9A). In one experimental setting Netrin1a-baits were preincubated with purified DraxinA before addition of the DCC prey. In the second set of experiments the Netrin1a-baits were incubated with a mixture of DraxinA and DCC prey proteins, and in the third set of experiments Netrin1a-baits have been preincubated with DCC preys followed by the addition of purified DraxinA as inhibitor. We did not observe a difference in the %-binding response between these 3 sets of experiments. These findings show that already formed DCC/Netrin1a complexes can be disrupted by the addition of DraxinA. These findings indicate that DraxinA has a higher affinity for Netrin1a than the Netrin receptor DCC.

Example 9 In Vivo Detection of the Draxin-Netrin Interaction in Zebrafish Embryos

To independently confirm the Draxin/Netrin1a interaction and to test whether both proteins are able to interact in vivo, we made use of transient protein overexpression experiments in zebrafish embryos. mRNAs encoding Draxin fused to superfolder GFP (Draxin-sfGFP) and Netrin1a tagged with mCherry (Netrin1a-mCherry) or superfolder GFP (Netrin1a-sfGFP) have been injected into one-cell stage zebrafish embryos. The distribution of the corresponding fluorophore tagged proteins has been analyzed in sphere stage zebrafish embryos (4 hours post fertilization) (FIG. 11A). At this developmental stage the extracellular space width between the cells is very large, ideally suited to visualize the localization of secreted proteins. Upon injection of mRNA encoding Draxin-sfGFP we observed an evenly distributed signal outside the cells in the extracellular milieu of 4 hpf zebrafish embryos (FIG. 11Ba). In contrast thereto, the distribution of Netrin1a-sfGFP was restricted to cell surface sub-domains (FIG. 11Bb).

When Draxin-sfGFP was coexpressed with Netrin1a-mCherry, Draxin-sfGFP re-located to Netrin1a-mCherry positive membrane associated densities (FIG. 11Bc). This indicated that localized Netrin1a-mCherry was able to capture diffusible Draxin-sfGFP.

The mRNA overexpression experiments showed that Draxin and Netrin1a are able to interact with each other in vivo. To further support this we used another strategy aiming to detect the distribution of endogenous Draxin interaction partners at developmental stages relevant for axon guidance decisions. From our binding assay with monomeric prey proteins we already had hints that the interaction between Draxin and Netrin is of high-affinity. Thus, we fused a netrin-binding fragment of the Draxin-ECD (aa209-284) to the human Fc region to generate an affinity probe. First, we tested this probe on zebrafish embryos from different developmental stages. After very gentle fixation the embryos were incubated with HEK293-6E cell supernatants containing the recombinant soluble Draxin_(aa209-284)-hFc protein.

Using an Alexa-Fluor 568 anti human IgG antibody to detect in situ bound Draxin_(aa209-284)-hFc we only detect a signal in close proximity to the floor plate (FIG. 12A, 12Ba). Because floor plate cells express Netrin1a and Netrin1b, we had indications that the signal detected by using the Draxin affinity probe indeed corresponds to in the extracellular space localized netrin. To prove this observation, we compared 48 hpf wt embryos with netrin1a and netrin1b double-knockdown embryos (FIG. 12Ba, 12Bb). In double-knockdown embryos the signal from the bound affinity probe was barely detectable compared to non-injected siblings, indicating that the Draxin_(aa209-284)-hFc probe indeed detected netrin. Taken together, the results from our mRNA overexpression and Draxin affinity probe experiments provide strong evidence that Draxin and Ntn1a are able to interact in vivo in zebrafish embryos.

Example 10 Human DRAXIN/Netrin-Signaling Network

To determine the binding specificity of DRAXIN/Netrin interactions, we carried out a pairwise binding screen between human DRAXIN and human Netrin family members. Except Netrin-5, we included all human Netrin family members consisting of two secreted γ-Netrins (Netrin-1 and Netrin-3) and one secreted (Netrin-4) and two GPI-linked β-Netrins (Netrin-G1 and Netrin-G2) in our binding study. Human DRAXIN and a 21 amino acid Netrin binding fragment derived thereof (SEQ ID NO.: 1) bound to Netrin-1 and Netrin-3 but not to human β-Netrin family members (FIG. 13).

These experiments confirm for human proteins the Draxin/γ-Netrin binding specificity within the Netrin family and showed that the human 21 amino acid DRAXIN fragment (SEQ ID NO.: 1), like its zebrafish counterpart (SEQ ID NOs.: 3), is sufficient for binding.

Example 11 Validation of the Draxin/Netrin1 Interaction by Surface Plasmon Resonance

Recombinant human Draxin was purchased from R&D systems. Biotinylated recombinant human UNC5B, Netrin1 and DCC were produced recombinantly using the described mammalian expression system (HEK293-6E). Biotinylated proteins were immobilised on the SA coated sensor chip and Draxin was injected sequentially in increasing concentrations (0 nM, 1.2 nM. 2.3 nM, 4.7 nM, 9.4 nM, 18.8 nM) for 3 min. Dissociation was allowed for 5 min in HBS-P. Binding was monitored and an interaction of Draxin to immobilised Netrin-1 was observed with a binding constant KD of approximately 20 to 100 nM. No binding of Draxin to UNC5B and DCC was detected (FIG. 14).

REFERENCES

-   Ahmed G, Shinmyo Y, Ohta K, Islam S M, Hossain M, Naser I B, Riyadh     M A, Su Y, Zhang S, Tessier-Lavigne M, Tanaka H: Draxin inhibits     axonal outgrowth through the netrin receptor DCC. J Neurosci. 2011     Sep. 28; 31(39):14018-23. -   Arakawa H: Netrin-1 and its receptors in tumorigenesis. Nat Rev     Cancer. 2004 December; 4(12):978-87. -   Bell C H, Healey E, van Erp S, Bishop B, Tang C, Gilbert R J,     Aricescu A R, Pasterkamp R J, Siebold C: Structure of the repulsive     guidance molecule (RGM)-neogenin signaling hub. Science. 2013 Jul.     5; 341(6141):77-80. -   Bushell K M, Söllner C, Schuster-Boeckler B, Bateman A, Wright G J:     Large-scale screening for novel low-affinity extracellular protein     interactions. Genome Res. 2008 April; 18(4):622-30. -   Castets M, Broutier L, Molin Y, Brevet M, Chazot G, Gadot N, Paquet     A, Mazelin L, Jarrosson-Wuilleme L, Scoazec J Y, Bernet A, Mehlen P:     DCC constrains tumour progression via its dependence receptor     activity. Nature. 2012; 482(7386):534-7. -   Delloye-Bourgeois C, Brambilla E, Coissieux M M, Guenebeaud C,     Pedeux R, Firlej V, Cabon F, Brambilla C, Mehlen P, Bernet A:     Interference with netrin-1 and tumor cell death in non-small cell     lung cancer. J Natl Cancer Inst. 2009 Feb. 18; 101(4):237-47. -   Durocher Y, Ferret S, Kamen A: High-level and high-throughput     recombinant protein production by transient transfection of     suspension-growing human 293-EBNA1 cells. Nucleic Acids Res. 2002     Jan. 15; 30(2):E9. -   Finci L I, Kruger N, Sun X, Zhang J, Chegkazi M, Wu Y, Schenk G,     Mertens H D, Svergun D I, Zhang Y, et al.: The Crystal Structure of     Netrin-1 in Complex with DCC Reveals the Bifunctionality of Netrin-1     As a Guidance Cue. Neuron 2014; 83(4):839-849. -   Fitamant J, Guenebeaud C, Coissieux M M, Guix C, Treilleux I,     Scoazec J Y, Bachelot T, Bernet A, Mehlen P: Netrin-1 expression     confers a selective advantage for tumor cell survival in metastatic     breast cancer. Proc Natl Acad Sci USA. 2008 Mar. 25; 105(12):4850-5. -   Glinka A, Wu W, Delius H, Monaghan A P, Blumenstock C, Niehrs C:     Dickkopf-1 is a member of a new family of secreted proteins and     functions in head induction. Nature. 1998 Jan. 22; 391(6665):357-62. -   Islam S M, Shinmyo Y, Okafuji T, Su Y, Naser I B, Ahmed G, Zhang S,     Chen S, Ohta K, Kiyonari H, Abe T, Tanaka S, Nishinakamura R,     Terashima T, Kitamura T, Tanaka H: Draxin, a repulsive guidance     protein for spinal cord and forebrain commissures. Science. 2009     Jan. 16; 323(5912):388-93. -   Haddick P C, Tom I, Luis E, Quiñones G, Wranik B J, Ramani S R,     Stephan J P, Tessier-Lavigne M, Gonzalez L C: Defining the Ligand     Specificity of the Deleted in Colorectal Cancer (DCC) Receptor. PLoS     One. 2014 Jan. 6; 9(1):e84823. -   Kastenhuber E, Kern U, Bonkowsky J L, Chien C B, Driever W, and     Schweitzer J: Netrin-DCC, Robo-Slit, and heparan sulfate     proteoglycans coordinate lateral positioning of longitudinal     dopaminergic diencephalospinal axons. J Neurosci 2009; 29:8914-8926. -   Kwan K M, Fujimoto E, Grabher C, Mangum B D, Hardy M E, Campbell D     S, Parant J M, Yost H J, Kanki J P, and Chien C B: The Tol2kit: a     multisite gateway-based construction kit for Tol2 transposon     transgenesis constructs. Dev Dyn 2007; 236:3088-3099. -   Leonardo E D, Hinck L, Masu M, Keino-Masu K, Ackerman S L,     Tessier-Lavigne M: Vertebrate homologues of C. elegans UNC-5 are     candidate netrin receptors. Nature. 1997 Apr. 24; 386(6627):833-8, -   Mann H H, Ozbek S, Engel J, Paulsson M, Wagener R: Interactions     between the cartilage oligomeric matrix protein and matrilins.     Implications for matrix assembly and the pathogenesis of     chondrodysplasias. J Biol Chem. 2004 Jun. 11; 279(24):25294-8. -   Mehlen P, Delloye-Bourgeois C, Chédotal A: Novel roles for Slits and     netrins: axon guidance cues as anticancer targets? Nat Rev Cancer.     2011 March; 11(3):188-97. -   Moore S W, Tessier-Lavigne M, Kennedy T E: Netrins and their     receptors. Adv Exp Med Biol. 2007; 621:17-31. -   Paradisi A, Creveaux M, Gibert B, Devailly G, Redoulez E, Neves D,     Cleyssac E, Treilleux I, Klein C, Niederfellner G, Cassier P A,     Bernet A, Mehlen P: Combining chemotherapeutic agents and netrin-1     interference potentiates cancer cell death. EMBO Mol Med. 2013     December; 5(12):1821-34. -   Paradisi A, Maisse C, Coissieux M M, Gadot N, Lépinasse F,     Delloye-Bourgeois C, Delcros J G, Svrcek M, Neufert C, Fléjou J F,     Scoazec J Y, Mehlen P: Netrin-1 up-regulation in inflammatory bowel     diseases is required for colorectal cancer progression. Proc Natl     Acad Sci USA. 2009 Oct. 6; 106(40):17146-51 -   Soliner C, Wright G J: A cell surface interaction network of neural     leucine-rich repeat receptors. Genome Biol. 2009; 10(9):R99. -   Suli A, Mortimer N, Shepherd I, and Chien C B: Netrin/DCC signaling     controls contralateral dendrites of octavolateralis efferent     neurons. J Neurosci 2006; 26:13328-13337. -   Xu K, Wu Z, Renier N, Antipenko A, Tzvetkova-Robev D, Xu Y,     Minchenko M, Nardi-Dei V, Rajashankar K R, Himanen J, et al.: Neural     migration. Structures of netrin-1 bound to two receptors provide     insight into its axon guidance mechanism. Science 2014; 344:     1275-1279. 

1. γ-Netrin-binding peptide comprising (i) the sequence EVMPTLDMALFDWTDYEDLKP (SEQ ID NO.: 1), (ii) the sequence DVAPTFNMALFDWTDYEDMRP (SEQ ID NO.: 2), (iii) the sequence EVMPTLDMTLFDWTDYEDMKP (SEQ ID NO.: 3), or (iv) a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.:1, 2 and/or 3, wherein said peptide has a length of up to 328 amino acids and is optionally fused to a heterologous peptide or polypeptide.
 2. The peptide according to claim 1, comprising any one of SEQ ID NO.: 7, SEQ ID NO.: 8, SEQ ID NO.: 9, SEQ ID NO.: 10, SEQ ID NO.: 11, SEQ ID NO.: 12, SEQ ID NO.: 13, SEQ ID NO.: 14 or SEQ ID NO.: 15 or a corresponding fragment of another species, preferably any one of SEQ ID NO.: 10, SEQ ID NO.: 11, SEQ ID NO.: 12, SEQ ID NO.: 13, SEQ ID NO.: 14, and SEQ ID NO.: 15, more preferably SEQ ID NO.: 15 or a corresponding fragment of another species, or a variant thereof having a sequence identity of at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% thereto.
 3. The peptide according to claim 1, which is fused to a heterologous peptide or polypeptide, preferably to a functional fragment of an immunoglobulin (Ig), preferably an Ig Fc fragment, more preferably a human Ig Fc fragment, more preferably a human IgG Fc fragment.
 4. The peptide according to any claim 1 for use in medicine, particularly human medicine.
 5. γ-Netrin-binding peptide comprising (i) the sequence EVMPTLDMALFDWTDYEDLKP (SEQ ID NO.: 1) (ii) the sequence DVAPTFNMALFDWTDYEDMRP (SEQ ID NO.: 2), (iii) the sequence EVMPTLDMTLFDWTDYEDMKP (SEQ ID NO.: 3), or (iv) a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.: 1, 2 and/or 3, which is optionally fused to a heterologous peptide or polypeptide, for use in the prevention or treatment of a condition associated with, accompanied by or mediated by pathologic, particularly increased, γ-Netrin expression or activity.
 6. The peptide for use according to claim 5, wherein said peptide has a length of up to 328, up to 324, up to 320, up to 300, up to 250, up to 200, up to 150, up to 100, up to 75 or up to 50 amino acids.
 7. The peptide for use according to claim 5, wherein said condition is a hyperproliferative disease, in particular a tumor disease, wherein said tumor disease is preferably selected from pancreatic cancer, colorectal cancer, breast cancer, and lung cancer.
 8. The peptide for use according to claim 5, wherein said condition is a cardiovascular disease, in particular atherosclerosis.
 9. The peptide for use according to claim 5, which is present as an active agent in a pharmaceutical composition together with at least one pharmaceutically acceptable carrier.
 10. The peptide for use according to claim 5, together with at least one additional therapeutically active agent, wherein the additional therapeutically active agent is particularly selected from the group consisting of decoy Netrin receptors, cytostatic agents, cytotoxic agents, statins, antihyperlipidemic agents, anti-coagulant agents, kinase inhibitors and angiogenesis modulators.
 11. Antibody or functional fragment or derivative thereof directed against Draxin, which inhibits binding of Draxin to at least one γ-Netrin, particularly Netrin1.
 12. Antibody or fragment or derivative thereof according to claim 11, which is directed against an epitope between amino acids 209-284, preferably between amino acids 226-257, more preferably between amino acids 232-252, of human Draxin.
 13. Antibody or functional fragment or derivative thereof directed against Netrin1, which inhibits binding of at least one γ-Netrin to at least one Netrin receptor, and which is directed against an epitope between amino acids 285-451 of human Netrin1 or a corresponding epitope in another species, such as an epitope located in the EGF1-3 domains of a zebrafish γ-Netrin.
 14. Antibody or fragment or derivative thereof according to claim 11 for use in medicine, particularly human medicine.
 15. Draxin-binding peptide comprising (i) at least 20 consecutive amino acids from the sequence KACDCHPVGAAGKTCNQTTGQCPC KDGVTGITCNRCANGYQQSRSPIAPCIKIPIAPP (SEQ ID NO.: 51) or (ii) a variant thereof having a sequence identity of at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% to SEQ ID NO.: 51, wherein said peptide has a length of up to 580 amino acids and is optionally fused to a heterologous peptide or polypeptide. 